si nc group (MedChemExpress)

MedChemExpress is a verified supplier

MedChemExpress manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

MedChemExpress si nc group
Si Nc Group, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/si+nc+group/SiRNA+Negative+Control/pm41489769-52-25-65
Average 95 stars, based on 52 article reviews

si nc group - by Bioz Stars, 2026-09

95/100 stars

Images

Control:

Article Title: GNB4 Silencing Promotes Pyroptosis to Inhibit the Development of Glioma by Activating cGAS-STING Pathway.
Article Snippet: The induction of immunogenic cell death is a promising therapeutic option for gliomas.. Pyroptosis is a type of programmed immunogenic cell death and its role in gliomas remains unclear.. Differentially expressed genes (DEGs) were obtained from GSE4290 and GSE31262 datasets.

Article Title: Overexpression of serum HMGB1 and IDO in esophageal squamous cell carcinoma patients: potential clinical auxiliary diagnostic markers and immunotherapeutic targets
Article Snippet: .. The control group, si-NC group, si-HMGB1 group and si-HMGB1+PMA group were cultured with RPMI 1640 medium containing 10% fetal bovine serum 6 hours later. si-HMGB1+PMA group was added with NF-κB signaling pathway activator Phorbol 12-myristate 13-acetate (PMA) (MedChemExpress, Shanghai, China), after transfection for 24 hours. .. After transfection for 48 hours, total protein was extracted with RIPA protein lysate (Solarbio, Beijing, China), and total protein concentration was determined with BCA protein concentration assay kit (Solarbio, Beijing, China).

Cell Culture:

Article Title: Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR/PI3K/AKT pathway.
Article Snippet: Bladder cancer (BC) is a heterogeneous disease, and pyrroline‐5‐carboxylate reductase 1 (PYCR1) can promote the proliferation and invasion of BC cells and accelerate BC progression.. In the present study, si‐PYCR1 was loaded into bone marrow mesenchymal stem cell (BMSC)‐derived exosomes (Exos) in BC.. First, PYCR1 levels in BC tissues/cells were assessed, and cell proliferation, invasion, and migration were evaluated.

Article Title: Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR/PI3K/AKT pathway
Article Snippet: Then, to the confocal Petri dishes, 200 μ l green-fluorescent probe was added (Actin-Tracker Green, cat. no. C1033, Beyotime Institute of Biotechnology) diluted with immunofluorescence staining secondary antibody diluent (1:200, cat. no. P0108, Beyotime Institute of Biotechnology) and incubated at 37°C for 30 min. After staining, the cells were washed with detergent for 5 min x 4 times, incubated with DAPI working solution for 10 min, and washed with PBS 4 times; the uptake rate (%) of Cy3-si-PYCR1 by T24 cells was observed under a laser confocal microscope (magnification, ×200, Leica GmbH). .. T24 cells and RT4 cells were seeded in 6-well plates, cultured until 80% confluence, and divided into the following groups based on different treatments: i) blank group, T24 cells or RT4 cells normally cultured for 48 h; ii) si-PYCR1 group and si-NC group, T24 cells transfected with the si-PYCR1 or the NC, respectively; iii) oe-PYCR1 group and oe-NC group, RT4 cells transfected with the oe-PYCR1 or the NC, respectively; iv) oe-PYCR1 + Vehicle group and oe-PYCR1 + CL-387785 group, RT4 cells treated with EGFR inhibitor CL-387785 (1 μ M, cat. no. HY-10325, MedChemExpress) or solvent (0.1% DMSO) for 2 h and transfected with oe-PYCR1. .. The dosage and method of use of CL-387785 were determined according prior to the study (data not shown); v) Exo group, T24 cells were cultured with Exos (100 ng/well) for 48 h; and vi) Exo-si-NC group and Exo-si-PYCR1 group, T24 cells were cultured with Exo-si-NC or Exo-si-PYCR1 (containing si-NC or si-PYCR1 100 ng/well) for 48 h. si-PYCR1, oe-PYCR1, and corresponding NCs used in transfection were synthesized by Shanghai GenePharma CO., Ltd.

Transfection:

Article Title: Nicotinamide improves the impaired extravillous trophoblast cell invasion induced by PM 2.5 exposure-associated increase of TNFα secretion through the ROS/NF-κB/FLT1 pathway.
Article Snippet: It has been well acknowledged that maternal exposure to fine particulate matters (PM2.5) might lead to poor pregnancy outcomes including the intrauterine growth restriction (IUGR) by interfering with the placental development.. Our previous studies have demonstrated that maternal PM2.5 exposure induces IUGR, accompanied with increased maternal circulating TNFα level and impaired extravillous trophoblast cells (EVTs) invasion in mice.. In this study, HTR8/SVneo cells, the immortalized human EVTs line, were used to assess effects and the underlying molecular mechanisms of nicotinamide on the impaired EVTs invasion.

Article Title: ELANE inhibits the progression of osteosarcoma via suppressing the CXCL12/CXCR4 axis.
Article Snippet: as the lungs [4].. OS originates from mesenchymal cells and has a poor prognosis because of its rapid invasive growth, early lung metastasis, and high recurrence rate [5, 6].. The current standard of care for OS is neoadjuvant chemotherapy combined with surgical resection, which includes preoperative chemotherapy, intraoperative radical resection, and postoperative chemotherapy [7].

Article Title: Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR/PI3K/AKT pathway.
Article Snippet: Bladder cancer (BC) is a heterogeneous disease, and pyrroline‐5‐carboxylate reductase 1 (PYCR1) can promote the proliferation and invasion of BC cells and accelerate BC progression.. In the present study, si‐PYCR1 was loaded into bone marrow mesenchymal stem cell (BMSC)‐derived exosomes (Exos) in BC.. First, PYCR1 levels in BC tissues/cells were assessed, and cell proliferation, invasion, and migration were evaluated.

Article Title: Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR/PI3K/AKT pathway
Article Snippet: Then, to the confocal Petri dishes, 200 μ l green-fluorescent probe was added (Actin-Tracker Green, cat. no. C1033, Beyotime Institute of Biotechnology) diluted with immunofluorescence staining secondary antibody diluent (1:200, cat. no. P0108, Beyotime Institute of Biotechnology) and incubated at 37°C for 30 min. After staining, the cells were washed with detergent for 5 min x 4 times, incubated with DAPI working solution for 10 min, and washed with PBS 4 times; the uptake rate (%) of Cy3-si-PYCR1 by T24 cells was observed under a laser confocal microscope (magnification, ×200, Leica GmbH). .. T24 cells and RT4 cells were seeded in 6-well plates, cultured until 80% confluence, and divided into the following groups based on different treatments: i) blank group, T24 cells or RT4 cells normally cultured for 48 h; ii) si-PYCR1 group and si-NC group, T24 cells transfected with the si-PYCR1 or the NC, respectively; iii) oe-PYCR1 group and oe-NC group, RT4 cells transfected with the oe-PYCR1 or the NC, respectively; iv) oe-PYCR1 + Vehicle group and oe-PYCR1 + CL-387785 group, RT4 cells treated with EGFR inhibitor CL-387785 (1 μ M, cat. no. HY-10325, MedChemExpress) or solvent (0.1% DMSO) for 2 h and transfected with oe-PYCR1. .. The dosage and method of use of CL-387785 were determined according prior to the study (data not shown); v) Exo group, T24 cells were cultured with Exos (100 ng/well) for 48 h; and vi) Exo-si-NC group and Exo-si-PYCR1 group, T24 cells were cultured with Exo-si-NC or Exo-si-PYCR1 (containing si-NC or si-PYCR1 100 ng/well) for 48 h. si-PYCR1, oe-PYCR1, and corresponding NCs used in transfection were synthesized by Shanghai GenePharma CO., Ltd.

Article Title: The role of autophagy in intervertebral disc degeneration and the regulation mechanism of AP-2α on autophagy.
Article Snippet: .. For subsequent functional assays, NP cells were grouped as follows: (1) si-NC group (cells transfected with negative control siRNA), (2) IDD+si-NC group (cells exposed to H2O2 and transfected with si-NC), (3) IDD+RA+si-NC group (cells exposed to H2O2 and transfected with si-NC, followed by treatment with the autophagy activator rapamycin [RA; MedChemExpress, USA]), (4) IDD+ 3MA+si-NC group (cells exposed to H2O2 and transfected with si-NC, followed by treatment with the autophagy inhibitor 3-methyladenine [3MA; MedChemExpress, USA]), (5) IDD+RA+si-AP-2α group (cells exposed to H2O2 and transfected with siRNA targeting AP-2α, followed by treatment with RA), and (6) IDD+ 3MA+si-AP-2α group (cells exposed to H2O2 and transfected with siRNA targeting AP-2α, followed by treatment with 3MA). .. All siRNAs (si-AP-2α and si-NC) were synthesized by Hanbio Biotechnology Co., Ltd. (Shanghai, China), and transfection was performed using LipofectamineTM 3000 (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol.

Recombinant:

Over Expression:

Plasmid Preparation:

Negative Control:

Solvent:

Article Title: Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR/PI3K/AKT pathway.
Article Snippet: Bladder cancer (BC) is a heterogeneous disease, and pyrroline‐5‐carboxylate reductase 1 (PYCR1) can promote the proliferation and invasion of BC cells and accelerate BC progression.. In the present study, si‐PYCR1 was loaded into bone marrow mesenchymal stem cell (BMSC)‐derived exosomes (Exos) in BC.. First, PYCR1 levels in BC tissues/cells were assessed, and cell proliferation, invasion, and migration were evaluated.

Article Title: Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR/PI3K/AKT pathway
Article Snippet: Then, to the confocal Petri dishes, 200 μ l green-fluorescent probe was added (Actin-Tracker Green, cat. no. C1033, Beyotime Institute of Biotechnology) diluted with immunofluorescence staining secondary antibody diluent (1:200, cat. no. P0108, Beyotime Institute of Biotechnology) and incubated at 37°C for 30 min. After staining, the cells were washed with detergent for 5 min x 4 times, incubated with DAPI working solution for 10 min, and washed with PBS 4 times; the uptake rate (%) of Cy3-si-PYCR1 by T24 cells was observed under a laser confocal microscope (magnification, ×200, Leica GmbH). .. T24 cells and RT4 cells were seeded in 6-well plates, cultured until 80% confluence, and divided into the following groups based on different treatments: i) blank group, T24 cells or RT4 cells normally cultured for 48 h; ii) si-PYCR1 group and si-NC group, T24 cells transfected with the si-PYCR1 or the NC, respectively; iii) oe-PYCR1 group and oe-NC group, RT4 cells transfected with the oe-PYCR1 or the NC, respectively; iv) oe-PYCR1 + Vehicle group and oe-PYCR1 + CL-387785 group, RT4 cells treated with EGFR inhibitor CL-387785 (1 μ M, cat. no. HY-10325, MedChemExpress) or solvent (0.1% DMSO) for 2 h and transfected with oe-PYCR1. .. The dosage and method of use of CL-387785 were determined according prior to the study (data not shown); v) Exo group, T24 cells were cultured with Exos (100 ng/well) for 48 h; and vi) Exo-si-NC group and Exo-si-PYCR1 group, T24 cells were cultured with Exo-si-NC or Exo-si-PYCR1 (containing si-NC or si-PYCR1 100 ng/well) for 48 h. si-PYCR1, oe-PYCR1, and corresponding NCs used in transfection were synthesized by Shanghai GenePharma CO., Ltd.

Review